Journal: Frontiers in Oncology

Article Title: Guanylate cyclase-C Signaling Axis as a theragnostic target in colorectal cancer: a systematic review of literature

doi: 10.3389/fonc.2023.1277265

Figure Lengend Snippet: GUCY2C-associated studies included in the systematic review.

Article Snippet: • Vaccine: Investigating a prime-boost approach involving a chimeric adenoviral vector (Ad5.F35) engineered to overcome pre-existing immunity, followed by recombinant Lm to enhance immune response toward the gastrointestinal cancer antigen GUCY2C. , • Vaccines: Ad-GUCY2C • Adenovirus expressing mouse GUCY2C1-429 fused to the influenza HA107-119 CD4+ T-cell epitope • Lm-GUCY2C and Lm-LacZ • mouse macrophage cell line J774A.1 (ATCC) • BALB/cJ mice , • In vitro infections • Immunizations • Ad5-neutralizing immunity studies • IFNγ ELISpot assay • Intracellular cytokine staining • In vivo tumor studies • Safety studies • Blood chemistry and cytokine analyses • Western blot , • Immunization with both heterologous Ad-GUCY2C and Lm-GUCY2C enhances CD8+ T-cell responses specific to GUCY2C and boosts antitumor immune activity. • Previous exposure to Ad5 restricts the effectiveness of Ad5.F35+Lm immunization protocols, whereas prior Lm exposure does not impose such limitations. • Alterations in the qualitative characteristics of the CD8+ T-cell population after a prime-boost vaccination regimen. • Lm-GUCY2C could potentially be employed to enhance GUCY2C-specific immune responses in patients undergoing clinical trials with adenovirus-based GUCY2C vaccines, aiming to prevent or manage recurrent GI cancer. , ( ) .

Techniques: Amplification, Expressing, Quantitative RT-PCR, Radioactivity, Activity Assay, Modification, Purification, Labeling, In Vivo, Mouse Assay, In Vitro, Clinical Proteomics, Viability Assay, Release Assay, Fluorescence, Staining, Immunofluorescence, Enzyme-linked Immunospot, Luciferase, Cell Culture, Activation Assay, Histopathology, Immunohistochemistry, Drug discovery, Western Blot, Isolation, Sequencing, Transduction, Variant Assay, Generated, Ex Vivo, Conjugation Assay, Flow Cytometry, Cytotoxicity Assay, Injection, Imaging, Mutagenesis, Protein-Protein interactions, Knock-Out, Transformation Assay, Plasmid Preparation, Control, Neutralization, Vaccines, Marker, Cell Isolation, Adoptive Transfer Assay, Transgenic Assay, Binding Assay, Cytotoxic T Lymphocyte Assay, Animal Model, Digital PCR, Gene Expression, Positron Emission Tomography, Comparison, Stability Assay, Immunopeptidomics, Recombinant, RNA Sequencing, Cloning, CRISPR, Chromatin Immunoprecipitation